Information & Resources Outline

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• 1.0 - Emergency - x7777 and Contacts

  • 1.1 - Emergency - x7777 and Contacts (Safety Information, Employee Handout, Emergency Assistance)

  • 1.2 - Generalized Biological Spill Protocol

• 2.0 - Read Me First

  • 2.1 - Read Me First (Orientation)

  • 2.2 - Empty

  • 2.3 - Empty

  • 2.4 - Biosafety Info Site Index

• 3.0 - Biosafety and Recombinant DNA

  • 3.1 - Empty

  • 3.2 - Training

    • 3.2.1 - Read Me First

      • 3.2.1.A - Read Me First (Training Folder)

    • 3.2.2 - CDC Biosafety Slide Show (General Biosafety Training)

      • 3.2.2.A - CDC Biosafety Slide Show (abridged)

      • 3.2.2.B - Training Documentation Sheet (Use this sheet to document all training)

    •  3.2.3 - Lab Equipment and Practices (Biosafety Training on Specific Subjects)

      • 3.2.3.A - Biological Safety Cabinets (BSCs; aka Tissue Culture Hoods)

        • 3.2.3.A.1 - BSC Movies (May need to download free Apple Quicktime)

          • 3.2.3.A.1.a - Shows airflow in a BSC

          • 3.2.3.A.1.b - What personal protective equipment to wear

          • 3.2.3.A.1.c - How to wipe down a BSC

          • 3.2.3.A.1.d - Turbulence caused by blocking BSC grills

          • 3.2.3.A.1.e - Setting up work from clean to contaminiated

        • 3.2.3.A.2 - Top BSC issues

        • 3.2.3.A.3 - Nuaire BSC Training Course

        • 3.2.3.A.4 - Selection, Installation and Use of BSCs, CDC/NIH/PHS

      • 3.2.3.B - Centrifuge Aerosol Biosafety

        • 3.2.3.B.1 - Fixed-angle rotor

        • 3.2.3.B.2 - Swinging-bucket rotor

      • 3.2.3.C - Biosafety Levels (BSL or BL) and Practices Summaries

        • 3.2.3.C.1 - BSL-1 to BSL-3 BMBL Summary for Labs

        • 3.2.3.C.2 - ABSL-1 & 2 BMBL Summary for Animal Labs

      • 3.2.3.D - Background information about exposures to aerosols

      • 3.2.3.E - Practical information about aerosols and procedures/equipment that generate them

      • 3.2.3.F - Setting up aspirators and where to order vacuum system filters

      • 3.2.3.G - Importing, exporting, interstate domestic shipping, etc.

      • 3.2.3.H - Regulated Medical Waste Definitions and Guidance

      • 3.2.3.J - Discussions about Tissue/Cell Culture Contamination

      • 3.2.3.K - The NIH rDNA Guidelines Explained

      • 3.4.3.L - Hazards of Chemical Powders movie

      • 3.2.3.M - Autoclave Safety

      • 3.2.3.N - Sharps Containers and Labeling

      • 3.2.3.O - Generalized Biological Spill Protocol, same doc as above
      • 3.2.3.P - Lab Hygiene and Personal Protective Equipment (PPE)

    • 3.2.4 - Animal User’s Safety Training

      • 3.2.4.A - Animal User’s Safety Training PowerPoint Slide Show

      • 3.2.4.B - Folder containing two folders that are support files for 3.2.4.A

      • 3.2.4.C - Fact Sheet on Lab Animal Allergies and Practices

      • 3.2.4.D - EPA Satellite Area Storage of Hazardous Waste

      • 3.2.4.E - UConn Health Center Occupational Animal Exposure Surveillance Program

      • 3.2.4.F - NIOSH Alert on Asthma Prevention

      • 3.2.4.G - The Guide for the Care and Use of Laboratory Animals

      • 3.2.4.H - Occupational Health and Safety in the Care and Use of Research Animals

      • 3.2.4.I - CDC Resources for Herpes B in handlers of macaques
      • 3.2.4.J - CDC Recommendations for Prevention and Therapy of Herpes B virus
      • 3.2.4.K - Documenting Occupational (human) hazards in Animal Work, e.g., Animal Care Protocols and ACC Safety Protocols.

  • 3.3 - BIO Laws, Guidelines and Summaries

    • 3.3.1 - Laws-BBP, Military, Bioterror, CTDPH

      • 3.3.1.A - Laws Affecting Biomedical Research

        • 3.3.1.A.1 - A partial list of laws that affect biomedical research

      • 3.3.1.B - Antibioterrorism

        • 3.3.1.B.1  - CDC Select Agent Regulations: Possession, Use, and Transfer of Select Agents and Toxins, 42 CFR Part 7 or on the web site.

        • 3.3.1.B.2 - USDA Select Agent Regulations: Agricultural Bioterrorism Protection Act of 2002; Possession, Use, and Transfer of Biological Agents and Toxins, 7 CFR Part 331 and 9 CFR Part 121

        • 3.3.1.B.3 - CDC/USDA Select Agent List

        • 3.3.1.B.4 - Bioresearch portions of the Patriot Act

        • 3.3.1.B.5 - 42 CFR Part 72.6 Regulation

        • 3.3.1.B.6 - CDC Select Agent Program (SAP)

          • 3.3.1.B.6.a  - SAP Helpful Resources (FAQs, exemptions, etc.)

        • 3.3.1.B.7 - USDA Select Agent Program

      • 3.3.1.C - Bloodborne Pathogen

        • 3.3.1.C.1 - OSHA Bloodborne Pathogen Regulations; Compliance Information

        • 3.3.1.C.2 - Bloodborne Pathogens Needlestick

        • 3.3.1.C.3 - OSHA Directive on Bloodborne Pathogens

        • 3.3.1.C.4 - OSHA Interpretation about Human Cells in Culture.

      • 3.3.1.D - State of Connecticut Biolaws

        • 3.3.1.D.1 - Connecticut Public Health Code19a-36-A (Requires registration for Infectious Agents)

        • 3.3.1.D.2 - Requirements for Institutions with BSL-3 Facilities

      • 3.3.1.E - Biosafety in Military and DOD Funded Labs

        • 3.3.1.E.1 - Biological Defense Safety Program Policy and Procedures

        • 3.3.1.E.2 - Biological Defense Safety Program Technical Requirements

    • 3.3.2 - NIH rDNA Guidelines, BMBL, Summaries

      • 3.3.2.A - Biosafety in Microbiological and Biomedical Laboratories, 4th Ed.,1999, CDC/NIH

        • 3.3.2.A.1 - 4th Edition Biosafety in Microbiological and Biomedical Laboratories or on the web site.

        • 3.3.2.A.2 - Appendix I Eye - Work with Toxins

      • 3.3.2.B - BSL-1 to BSL-3 Summary; same as above in training   

      • 3.3.2.C - Animal BSL-1 & ABSL-2 Summary; same as above in training

      • 3.3.2.D - NIH rDNA Guidelines (nihG)

        • 3.3.2.D.1 - NIH Guidelines for Research Involving Recombinant DNA Molecules or NIH Office of Biotechnology Activities (OBA)

        • 3.3.2.D.2 - NIH Lab Safety Monograph 1979

        • 3.3.2.D.3 - NIH Guidelines for Research Involving Recombinant DNA Molecules (nihG)

        • 3.3.2.D.4 - The NIH rDNA Guidelines Explained; same as above in training

      • 3.3.2.E - Canadian – Lab Biosafety Guidelines
        Canadian Office of Laboratory Security Web Site
        Fact sheets (MSDSs) for infectious agents

      • 3.3.2.F - WHO Lab Biosafety Manual from Web Site

      • 3.3.2.H - Empty

  • 3.4 - Bloodborne Pathogens (BBP), UConn Health Center

    • 3.4.1 - BBP Exposure Control Plan

    • 3.4.2 - BBP Rule Summary 2002

    • 3.4.3 - BBP Related + Needles

      • 3.4.3.A - Empty

      • 3.4.3.B - Monoject Instructions

      • 3.4.3.C - Safety Syringes (Excel file)

    • 3.4.4 - BBP Web training – Initial and Annual - call x2723 for information.

  • 3.5 - Connecticut State Registration

    • 3.5.1 - Read Me First

    • 3.5.2 - Forms

      • 3.5.2.A - Read Me First

      • 3.5.2.B - Lab Registration Instructions

      • 3.5.2.C - Lab Registration Application (Word doc)

      • 3.5.2.D - List of Notaries

    • 3.5.3 - State Lab Checklist

    • 3.5.4 - State Animal Lab Checklist

    • 3.5.5 - Biohazard Spill Outside the Biological Safety Cabinet (Biological Hood)

  • 3.6 - Risk Groupings of Organisms - Risk Group and Biosafety Level Definitions; Risk Groups for Bacteria, Fungi, Viruses, Parasites

    • 3.6.1 - Miscellaneous

      • 3.6.1.A - Study of pathogenicity of various lab strains of E.coli

      • 3.6.1.B - NCI biosafety for use of Adenovirus

• 4.0 - Chemical Safety

  • 4.1 - UConn Health Center Chemical Guidelines

    • 4.1.1 - Chemical Hygiene Plan 2003

    • 4.1.2 - UConn Health Center chemical Carcinogen Guidelines

    • 4.1.3 - Antineoplastic & Hazardous Drug Safety

  • 4.2 - CHEMLaws

    • 4.2.1 - OSHA Hazardous Chemical Regulations: Occupational exposure to hazardous chemicals in laboratories. – 29 CFR Part 1910.1450

  • 4.3 - ChemWaste

    • 4.3.1 - Hazard Chemical Waste EPA List

  • 4.4 - Empty

  • 4.5 - Prudent Practices Book

    • 4.5.1 - Prudent Practices in the Laboratory: Handling and Disposal of Chemicals

  • 4.6 - ChemMovie

    • 4.6.1 - Hazards of Chemical Powders Movie; same as above

• 5.0 - Transport - Shipping

  • 5.1 - Overview

    • 5.1.1 - Read Me First

  • 5.2 - Shipping Permits and Regulations

    • 5.2.1 - CDC Instructions and Link to Import Permit (infectious to humans)

      • 5.2.1.A - DHHS Regulations

        • 5.2.1.A.1 - Transport

          • 5.2.1.A.1.a - 42 CFR 72.1-6

        •  5.2.1.A.2 - Biowarfare

          • 5.2.1.A.2.a - 42 CFR Part 1003: Possession, Use, and Transfer of Select Agents and Toxins; Final Rule

          • 5.2.1.A.2.b - Current DHHS and USDA Listed Biological Agents

    • 5.2.2 - Department of Transportation

      • 5.2.2.A - Read Me First

      • 5.2.2.B - Infectious Disease Interstate Transport

    • 5.2.3 - USDA Permit

      • 5.2.3.A - Import and Interstate Transport Permit Forms

        • 5.2.3.A.1 - Empty

        • 5.2.3.A.2 - NCIE (USDA National Center for Import and Export) Instructions Interpretations

          • 5.2.3.A.2.a - NCIE Instructions and permit forms

          • 5.2.3.A.2.b - Guidelines for Products that do not Require an Import Permit (USDA)

      • 5.2.3.B - The USDA Regulations

        • 5.2.3.B.1 - Transport Permits

          • 5.2.3.B.1.a - Importing and Interstate Transfer

        • 5.2.3.B.2 - Biowarfare

          • 5.2.3.B.2.a - Agricultural Bioterrorism Protection
            Act of 2002

          • 5.2.3.B.2.b - Select Agents and Toxins List

  • 5.3 - Exporting

    • 5.3.1 - Don't Let This Happen To You (Don't export the wrong thing to the wrong person)

    • 5.3.2 - Export Licensing Information

• 6.0 - Waste

  • 6.1 - Biomed Waste

    • 6.1.1 - UConn Health Center Medical Waste Rules

  • 6.2 - Waste Laws

    • 6.2.1 - Connecticut General Statutes Medical Waste

    • 6.2.2 - Hazard Chemical Waste EPA List

• 7.0 - Electronic Hazard Symbols

  • 7.1 - Electronic Hazard Symbols (Word doc)

  • 7.2 - Electronic Hazard Symbols